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Year : 2021  |  Volume : 8  |  Issue : 2  |  Page : 57-60

Evaluation of fungal elements in dystrophic nails using Chicago sky blue stain

Department of Dermatology, Venereology and Leprology, Victoria Hospital, Bengaluru, Karnataka, India

Correspondence Address:
Sowmya S Aithal
4753/A, Sindhoora, Opposite Jyothi IVF Centre, Dattagalli 3rd Stage, Mysore 570022, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ijdpdd.ijdpdd_14_21

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Background: Dystrophic nails represent an alteration in nail morphology that can occur due to various disorders. Although KOH is used commonly in the diagnosis, the sensitivity and specificity of KOH are less. Chicago sky blue (CSB) stain when used with KOH provides color contrast, to aid the detection of fungal elements, and is a simple and inexpensive method. Materials and Methods: Nail scrapings from the surface and subungual areas and nail clippings were examined with 40% KOH, CSB stain, and also subjected to fungal culture. Results: The detection rate of fungal elements in dystrophic nails in our hospital-based study was 46.5%. The sensitivity of KOH was 36.36% and CSB staining was 66.6% using fungal culture as a gold standard. Conclusion: CSB stain serves as a useful, simple, and rapid method for detecting the presence of fungal elements.

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